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Vitrification and Warming Media. Modifications in cryopreservation methods over the past few years may be responsible for improved survival of cryo-preserved mature oocytes. Cryoprotective perfusion is the core procedure of Alcor’s human cryopreservation protocol. Here, we present a vitrification method for mouse embryos that has been successfully used at our center. Without the introduction of a vitrification solution, extensive damage to the brain should be expected. The cryopreservation solution contains ethylene glycol instead of DMSO to minimize the toxicity to embryos 5. For clinical cryopreservation, vitrification usually requires the addition of cryoprotectants prior to cooling. ... resulted in a basic protocol that is still in vast use today. REFERENCES 1. Vitrification of tissue engineered constructs was first reported by Lilia Kuleshova, who also was the first scientist to achieve vitrification of oocytes, which resulted in live birth in 1999. It also contains Ficoll and sucrose for prevention of … A closed system supports the development competence of human embryos after vitrification. Reproductive Biology and Endocrinology 2013; 11:41. Please Kitazato media are the most versatile option for cryopreservation in your laboratory. These cryopreserved resources have been successfully used to rederive next generation pups using embryo transfer and intracytoplasmic sperm injection (ICSI). Vitrification has been used as an effective, simple and inexpensive protocol for cryopreservation of sperm (Endo et al., 2012), oocytes (Cai et al., 2005) and embryos (Ali and Shelton, 1993). In this chapter, we describe in detail protocols for rat embryo vitrification and sperm cryopreservation followed by pup rederivation using the ICSI procedure and embryo transfer. 2. Oocyte, zygote, embryo and blastocyst freezing by vitrification method for cryopreservation have been used for many years beside sperms preservation. IVF protocol for sperm samples frozen in vials and MTG straws (Sep 2018) Laser-Assisted IVF (Sep 2018) Mouse intracytoplasmic sperm injection (ICSI) (Nov 2016) A simple and efficient vitrification procedure for cryopreservation of mouse embryos in straws (Sep 2018) Harvest and cryopreservation of in vivo derived mouse embryos (Sep 2018) Vitrification (Ultra-rapid Cryopreservation) of Embryos as well as Oocytes Vitrification is an alternative method to embryo and oocyte cryopreservation which often has been recently referred to as a revolutionary technique; on the other hand, the very first successful embryo vitrification was published around typically the middle of the eighties. The new Rapid-i carrier is an effective system for human embryo vitrification at both the blastocyst and cleave stage. Hashimoto S et al. Desai et al. Key Features Same protocol for oocytes and/or embryos Simple and easy-to-follow protocol Ready-to-use DMSO free media Availability This product is available for sale in Europe, the U.S.A, Canada, China, Australia as well as selected countries in the rest of the world. MediCult Vitrification Cooling MediCult Vitrification Cooling is for vitrification of human oocytes and/or embryos. cryopreservation technique and may vary by time after thaw (8). Vitrification is a cryopreservation technique that leads to a glass-like solidification. Reduce your costs by using the same media for vitrification and warming of oocytes and embryos, in all their stages of development, from Pronucleus to Blastocyst.